sciencell  內(nèi)皮細(xì)胞培養(yǎng)基（ECM）

Endothelial Cell Medium(ECM)

Catalog Number: 1001

Product Description

Endothelial cell medium (ECM) is a complete medium designed for optimal growth of normal human  microvascular endothelial  cells  in vitro.  It is a sterile, liquid medium  which  contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in  an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human microvascular endothelial cells in vitro.

Components

ECM consists of 500 ml of basal medium, 25 ml of fetal bovine serum (FBS, Cat. No. 0025), 5 ml of  endothelial cell growth supplement (ECGS, Cat. No. 1052) and 5 ml of  penicillin/streptomycin solution (P/S, Cat. No. 0503). 

Product Use

ECM is for research use only.  It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Store the basal medium at 4℃, the ECGS, the FBS and the P/S solution at -20℃. Protect from light. 

Shipping

Gel ice.

Prepare for use

Thaw  ECGS, FBS and P/S solution at 37℃.  Gently tilt the  ECGS tube several times during thawing  to help the contents dissolve.  Make sure the contents of the supplement are compley dissolved into solution before adding to the medium. Rinse the bottle and tubes with 70% ethanol, and then wipe to remove excess. Remove the cap, being careful not to touch the interior threads with fingers. Add ECGS, FBS and P/S solution into basal medium in a sterile field, mix well and then the reconstituted medium is ready for use. Since several components of this medium are light-labile, it is recommended that the medium not be exposed to light for lengthy periods of time. If the medium is warmed prior to use, do not exceed 37℃. When stored in the dark at 4℃, the reconstituted medium is stable for one month.

Caution:

If handled improperly, some components of the medium may present a health hazard. Take appropriate precautions when handling it, including the wearing of protective clothing and eyewear. Dispose of properly.

美國(guó)ScienCell研究實(shí)驗(yàn)室成立于1999年，公司總部位于美國(guó)加州的圣地亞哥。主要致力于實(shí)驗(yàn)室科研用原代細(xì)胞、原代細(xì)胞培養(yǎng)基、原代細(xì)胞無血清培養(yǎng)基、干細(xì)胞、干細(xì)胞培養(yǎng)基、干細(xì)胞無血清培養(yǎng)基的研究和開發(fā)，在擁有眾多客戶。在國(guó)內(nèi)銷售8年來，很多老師應(yīng)用其產(chǎn)品發(fā)表了高質(zhì)量的SCI文章，且有著的文獻(xiàn)引用率。憑借著嚴(yán)格的質(zhì)控和的產(chǎn)品品質(zhì)，深受廣大科研工作者的信賴。
       ScienCell研究實(shí)驗(yàn)室生產(chǎn)的原代細(xì)胞、原代細(xì)胞培養(yǎng)基都經(jīng)過了嚴(yán)格的質(zhì)量控制，細(xì)胞純度可達(dá)98％。其中包括21種人體正常細(xì)胞系統(tǒng)，90多種不同細(xì)胞類型。大多數(shù)細(xì)胞在唯有ScienCell實(shí)驗(yàn)室能夠成功分離，產(chǎn)品質(zhì)量過硬。確保了實(shí)驗(yàn)結(jié)果的重復(fù)性和連貫性。

內(nèi)皮細(xì)胞培養(yǎng)基（ECM）是專門為正常人類微血管內(nèi)皮細(xì)胞體外培養(yǎng)設(shè)計(jì)的*適于其生長(zhǎng)的*培養(yǎng)基。內(nèi)皮細(xì)胞培養(yǎng)基（ECM）是經(jīng)滅菌的液體培養(yǎng)基，包含必需和非必需氨基酸、維生素、有機(jī)和無機(jī)化合物、***、生長(zhǎng)因子、微量礦物質(zhì)和低濃度胎牛血清(5%)。內(nèi)皮細(xì)胞培養(yǎng)基（ECM）緩沖體系為重碳酸鹽，在含5%CO2的細(xì)胞培養(yǎng)箱中平衡后pH值為7.4。內(nèi)皮細(xì)胞培養(yǎng)基（ECM）的配方能夠選擇性的促進(jìn)正常人類微血管內(nèi)皮細(xì)胞體外培養(yǎng)中的增殖和生長(zhǎng)，并為其達(dá)到*理想營(yíng)養(yǎng)平衡狀態(tài)提供數(shù)量上和質(zhì)量上的保證。
      內(nèi)皮細(xì)胞培養(yǎng)基（ECM）包含500ml基礎(chǔ)培養(yǎng)基，25ml胎牛血清(FBS,目錄編號(hào)0025)，5ml內(nèi)皮細(xì)胞生長(zhǎng)添加物(ECGS,目錄編號(hào)1052)，和5 ml青霉素/鏈霉素溶液(P/S,目錄編號(hào)0503)

sciencell  內(nèi)皮細(xì)胞培養(yǎng)基（ECM）訂購(gòu)說明：

1、絕大部分產(chǎn)品備有現(xiàn)貨，一般情況下都能訂貨當(dāng)日發(fā)貨。 
2、部分非常用產(chǎn)品，需提前1－2日預(yù)訂，海外期貨則需要提前3-6周預(yù)訂。 
3、部分產(chǎn)品價(jià)格會(huì)因貨期、批次等因素發(fā)生變化，若有變動(dòng)以訂貨當(dāng)日新價(jià)格為準(zhǔn)。 
4、每日訂單截止時(shí)間為16點(diǎn)整，部分城市可到17點(diǎn)整，因超過截止時(shí)間造成當(dāng)日不能發(fā)貨的將于次日安排發(fā)貨。
5、請(qǐng)辦理完貨款后，將收據(jù)、底單等連同收貨人的地址、姓名、等以傳真、、短信、等形式通知我們。

sciencell  內(nèi)皮細(xì)胞培養(yǎng)基（ECM）運(yùn)輸說明：

極低溫產(chǎn)品：極低溫產(chǎn)品運(yùn)輸過程中加裝干冰運(yùn)輸。用干冰把產(chǎn)品包裹起來，再用泡沫盒密封，膠帶層層粘住泡沫盒，放入索萊寶的箱子，然后交付給合作快遞，安全快速高效放心的送客戶的手中，保證產(chǎn)品的性質(zhì)穩(wěn)定如一，為您的實(shí)驗(yàn)保駕護(hù)航。
低溫產(chǎn)品：低溫產(chǎn)品運(yùn)輸過程中加裝索萊寶冰袋運(yùn)輸。事先用冰袋把產(chǎn)品包裹起來，再使用泡沫盒密封，用膠帶嚴(yán)實(shí)密封泡沫盒，再放入索萊寶的箱子（保溫效果少可以持續(xù)一周），然后交付給合作快遞，安全快速高效放心的送客戶的手中，保證產(chǎn)品的性質(zhì)穩(wěn)定如一，為您的實(shí)驗(yàn)保駕護(hù)航。
常溫產(chǎn)品：常溫產(chǎn)品運(yùn)輸過程中無需加冰或者特殊包裝。產(chǎn)品由公司庫房人員快速配貨，準(zhǔn)確快速高效的快遞保證產(chǎn)品快速送達(dá)您的手中。