DMEM高糖 細(xì)胞培養(yǎng) solarbio

DMEM是現(xiàn)今細(xì)胞培養(yǎng)中比較常見的培養(yǎng)基之一，dmem培養(yǎng)基是建立在MEM培養(yǎng)基的基礎(chǔ)上研究出來的，它與MEM培養(yǎng)基相比較，DMEM培養(yǎng)基中加大了MEM培養(yǎng)基中各種成分的用量，下文主要介紹一下自己配制DMEM的方法。

1 制備新鮮三蒸水或Millipore超純水。

2 稱取所需量的干粉培養(yǎng)基，加入約終體積一半的三蒸水中;若配制一個(gè)包裝的培養(yǎng)液，在將整個(gè)包裝的干粉倒入三蒸水后，需用水洗包裝袋內(nèi)面2次，倒入培養(yǎng)液中，以保證所有干粉都溶解成培養(yǎng)液。磁力攪拌或人工攪拌使之*溶解。

3 根據(jù)包裝袋上的要求補(bǔ)加所需量的碳酸氫鈉;根據(jù)實(shí)驗(yàn)需要，添HEPES(5-20mmol/L)、谷氨酰胺和其他特殊物質(zhì)。

4 加水定容到終體積。

5 必要時(shí)用1 mol/L 鹽酸和1 mol/L 氫氧化鈉調(diào)節(jié)pH。

6 用無菌0.22um濾膜過濾除菌，分裝于無菌血清瓶中，4℃冰箱保存。

配制好的培養(yǎng)液用前加入100U/mL青霉素和100U/mL鏈霉素，并根據(jù)需要加入血清(5%-20%)。 DMEM培養(yǎng)基應(yīng)用廣泛?；贒MEM培養(yǎng)基中氨基酸、微生物含量豐富，并具有糖酵解中的丙酮酸和微量Fe離子，因此在細(xì)胞培養(yǎng)、疫苗生產(chǎn)等方面的應(yīng)用為常見。

相關(guān)文獻(xiàn)：

《Effects of C-myc gene silencing on interleukin-1β-induced rat chondrocyte cell proliferation, apoptosis and cytokine expression》 作者:Jian Zou，Xiao-Lin Li，Zhong-Min Shi，Jian-Feng Xue 期刊:Journal of Bone and Mineral Metabolism 影響因子:2.219 PMID:28616752
《Hematopoietic Substrate-1-Associated Protein X-1 Regulates the Proliferation and Apoptosis of Endothelial Progenitor Cells Through Akt Pathway Modulation》 作者:XIN-BIN GUO,XIN DENG,YING WEI 期刊:Wiley Online Library 影響因子: PMID:29139175
《c-Myc induced upregulation of long non-coding RNA SNHG16 enhances progression and carcinogenesis in oral squamous cell carcinoma》 作者:Shangfeng Li, Shengkai Zhang， Jie Chen 期刊:Cancer gene therapy 影響因子:4.681 PMID:30607006
《Molecular characterization of Cyprinid herpesvirus 3 encoded viral interleukin10》 作者:Ping Ouyang，Ruixue Yang，Lizi Yin，Yi Geng，Weiming Lai，Xiaoli Huang，Defang Chen，Jing Fang，Zhengli Chen，Li Tang，Min He，Chao Huang，Wentao Liu，Kaiyu Wang 期刊:Fish & Shellfish Immunology 影響因子:3.298 PMID:30926478
《Exosomes from microRNA-199-3p-modified adipose-derived stem cells promote proliferation and migration of endothelial tip cells by downregulation of semaphorin 3A》 作者:Lingjuan Du, Guojian Li, Yong Yang, Guokai Yang, Jia Wan, Zhenhuan Ma, Yi Hou 期刊:International Journal of Clinical and Experimental Pathology 影響因子:0.205 PMID:
《Sphingomyelin synthase 2 promotes H2O2-induced endothelial dysfunction by activating the Wnt/β-catenin signaling pathway》 作者:Panpan Zhang,Lingyue Hua,Huan Hou, Xingyue Du,Zhiqiang He, Menghan Liu, Xiaojuan Hu, Nianlong Yan 期刊:International Journal of Molecular Medicine 影響因子:2.928 PMID:30272329
《Identification of Anticancer Compounds in Gallnuts Through PCA-constructed Secondary Metabolite Map》 作者:Jiayu Gao, Xiao Yang, Jiangxia Hu and Weiping Yin 期刊:International Journal of Pharmacology 影響因子:0.754 PMID:
《Effect of Hypoxia on the Muscle Fiber Switching Signal Pathways CnA/NFATc1 and Myostatin in Mouse Myocytes》 作者:Lixin Li,Juan Wang,Yun Bai,Jingwei Li,Xiuju Yu,Xiaomao Luo,Zhiwei Zhu,Xiaoyan He,Yanjun Dong,Hongquan Li,Haidong Wang 期刊:Acta Histochemica 影響因子:4.239 PMID:31047685
《Knockdown of USP28 enhances the radiosensitivity of esophageal cancer cells via the c‐Myc/hypoxia‐inducible factor‐1 alpha pathway》 作者:Zhang Weili， Liu Zhikun，Wang Jianmin， Tian Qingbao 期刊:Journal of Cellular Biochemistry 影響因子:3.448 PMID:30206969
《HDAC2, but not HDAC1, regulates Kv1.2 expression to mediate neuropathic pain in CCI rats》 作者:Zhihua Li, Yanyan Guo, Xiuhua Ren, Lina Rong, Minjie Huang, Jing Cao, Weidong Zang 期刊:Neuroscience 影響因子:3.244 PMID:31022463
《Differentially expressed genes of HepG2 cells treated with gecko polypeptide mixture》 作者:Yi-Meng Duan, Ying Jin, Meng-Li Guo, Leng-Xin Duan, and Jian-Gang Wang 期刊:Journal of Cancer 影響因子:3.182 PMID:30087713
《青蒿琥酯對(duì)高糖條件下血管內(nèi)皮細(xì)胞ICAM-1和MMP-9表達(dá)的影響》 作者:葛朋飛,姜 濤,宗 瑤,楊雪嬌,馬玉娜,王云霄,孫 哲,曹子群 期刊:Hans Journal of Surgery 影響因子: PMID:
《MiR-182-5p and miR-96-5p increased hepatocellular carcinoma cell mobility, proliferation and cisplatin resistance partially by targeting RND3》 作者:Shiming Yang, Zhi Chen, Daguang Fan, Rui Zhang, Yuhong Zhang and Shusheng Wu 期刊:RSC Adv. 影響因子:2.789 PMID:
《A Host Factor GPNMB Restricts Porcine Circovirus Type 2 (PCV2) Replication and Interacts With PCV2 ORF5 Protein》 作者:Kangkang Guo, Lei Xu, Mengmeng Wu, Yufeng Hou, Yanfen Jiang, Jiangman Lv, Panpan Xu, Zhixin Fan, Ruiqi Zhang, Fushan Xing and Yanming Zhang 期刊:Frontiers in Microbiology 影響因子:4.019 PMID:30671053
《Effect of acute exposure to PFOA on mouse liver cells in vivo and in vitro》 作者:Xinmou Wu，Minqing Li，Zhao Yang，Min Su，Bin Yang 期刊:Environmental Science and Pollution Research 影響因子:2.8 PMID:28887612
《Lipid raft localization of epidermal growth factor receptor alters matrix metalloproteinase-1 expression in SiHa cells via the MAPK/ERK signaling pathway》 作者:Zongfeng Zhang, Lina Wang ,Juan Du ,Yuanbo Li, Huilun Yang, Chenxi Li, Hui Li, Haiyang Hu 期刊:Oncology Letters 影響因子:0.237 PMID:28101233
《Trophoblast glycoprotein promotes pancreatic ductal adenocarcinoma cell metastasis through Wnt/planar cell polarity signaling》 作者:Ping He, Shuheng Jiang, Mingze Ma ,Yang Wang, Rongkun Li, Fang Fang, Guangang Tian ,Zhigang Zhang 期刊:Molecular Medicine Reports 影響因子:1.922 PMID:25738465
《High Expression of IL-4R Enhances Proliferation and Invasion of Hepatocellular Carcinoma Cells》 作者:C Guo, Y Ouyang, J Cai, L Xiong 期刊:The International Journal of Biological Markers 影響因子:1.449 PMID:28665449
《Effect of Mst1 overexpression on the growth of human hepatocellular carcinoma HepG2 cells and the sensitivity to cisplatin in vitro》 作者:C Xu, C Liu, W Huang, S Tu, F Wan 期刊:Acta Biochim Biophys Sin 影響因子:2.224 PMID:23419720
《TINAGL1 promotes hepatocellular carcinogenesis through the activation of TGF-β signaling-medicated VEGF expression》 作者:L Sun, Z Dong, H Gu, Z Guo, Z Yu 期刊:Cancer Management and Research 影響因子:3.702 PMID:30697069
《Selective anticancer activity of the novel steroidal dihydropyridine spirooxindoles against human esophageal EC109 cells》 作者:Xiaoli Shi,Tiantian Qin,Weihua Liu,Xueyan Zhang,Leilei Li,Junfeng Huo,Kairui Zhou,Dongxiao Yang,Yanling Zhang,Cong Wang 期刊:Biomedicine & Pharmacotherapy 影響因子:2.068 PMID:
《Gastroprotective effect of gamma-aminobutyric acid against ethanol-induced gastric mucosal injury》 作者:Min Xie,Haihong Chen,Shaoping Nie,Wei Tong,Junyi Yin,Mingyong Xie 期刊:Chemico-Biological Interactions 影響因子:3.296 PMID:28456544
《共軛亞油酸異構(gòu)體對(duì)胰島素抵抗脂肪細(xì)胞消耗葡萄糖的影響》 作者:劉曉華， 劉萍， 李海星， 李超波， 曹郁生 期刊:南昌大學(xué)學(xué)報(bào)(理科版), 影響因子:1.187 PMID:
《蜂膠中 ４ 種黃酮對(duì) ＨｅｐＧ２ 細(xì)胞胰島素抵抗的改善作用》 作者:梁霞麗　趙亞周　張紅城　彭文君 期刊:中國農(nóng)業(yè)科技導(dǎo)報(bào) 影響因子:1.439 PMID:
《Upregulated lncRNA small nucleolar RNA host gene 1 promotes 1‐methyl‐4‐phenylpyridinium ion‐induced cytotoxicity and reactive oxygen species production through miR‐15b‐5p/GSK3β axis in human dopaminergic SH‐SY5Y cells》 作者:Na Xie， Jinxing Qi， Shuang Li， Jianzhong Deng， Yuan Chen， Yajun Lian 期刊:Journal of Cellular Biochemistry 影響因子:3.448 PMID:30302821
《Label?free microfluidic chip for the identification of mesothelial cell clusters in pleural effusion》 作者:Lili Zhao, Meng Zhao, Yu Yang, Yajun Gu ,Fang Zheng ,Xuan Wang ,Zhiyuan Zheng, Xuguo Sun 期刊:Oncology Letters 影響因子:1.871 PMID:30944642
《Emodin Attenuates Lipopolysaccharide-Induced Injury via Down-Regulation of miR-223 in H9c2 Cells》 作者:Yuping Yang, Zijun Jiang, Dong Zhuge 期刊:International Heart Journal 影響因子:2.226 PMID:30745529
《Downregulation of lncRNA NEAT1_2 radiosensitizes hepatocellular carcinoma cells through regulation of miR‐101‐3p/WEE1 axis》 作者:Xin Chen , Nuobei Zhang 期刊:Cell Biology International 影響因子:2.127 PMID:30488993
《miR?9 depletion suppresses the proliferation of osteosarcoma cells by targeting p16》 作者:Song Gao, Jianchao Wang, Shujian Tian, Jianping Luo 期刊:International Journal of Oncology 影響因子:3.571 PMID:31081054
《Long Non-Coding RNA NIFK-AS1 Augments the Oncogenicity of Hepatocellular Carcinoma by Regulating miR-497/BIRC5 Axis》 作者:Yajing Feng,Lu Sun,Yajuan Feng,Zhixian Guo 期刊:SSRN 影響因子: PMID:
《Sulfated modification enhanced the antioxidant activity of Mesona chinensis Benth polysaccharide and its protective effect on cellular oxidative stress》 作者:Lixin Huang,Min Huang,Mingyue Shen,Pingwei Wen,Ting Wu,Yuzhen Hong,Qiang Yu,Yi Chen,Jianhua Xie 期刊:International Journal of Biological Macromolecules 影響因子:4.784 PMID:31254574
《miR-185 silencing promotes the progression of atherosclerosis via targeting stromal interaction molecule 1》 作者:Ming Fang, Yanfei Li, Yingbiao Wu, Zhongping Ning, Xuejun Wang & Xinming Li 期刊:Cell Cycle 影響因子:3.259 PMID:30784343
《Insulin-like growth factor 2 mRNA binding protein 2 promotes aerobic glycolysis and cell proliferation in pancreatic ductal adenocarcinoma via stabilizing GLUT1 mRNA》 作者:Shan Huang , Zheng Wu , Yunyun Cheng , Wenzhen Wei  Linlin Hao 期刊:Acta Biochimica et Biophysica Sinica 影響因子:2.502 PMID:31089713
《Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression》 作者:Longfei Xiao ， Liangli Song，  Junjie Hu ， Quanwei Zhang ， Yuting Jiang ， Hongwei Duan ， Yong Zhang， Xingxu Zhao ， Yongli Hua 期刊:Journal of Cellular Physiology 影響因子:4.522 PMID:30671954
《Biological characteristics of heat shock protein 90 in human liver cancer cells》 作者:Li Qin, Haixin Huang, Jian Huang, Guodong Wang, Jinxin Huang, Xintian Wu, Jinzhuan Li, Weili Yi, Longjing Liu, and Dongning Huang 期刊:American Journal of Translational Research 影響因子:3.266 PMID:31105855
《Effect of Artesunate on the Expression of ICAM-1 and MMP-9 in Vascular Endothelial Cells under High Glucose Condition》 作者:Pengfei Ge, Tao Jiang, Yao Zong, Xuejiao Yang, Yuna Ma, Yunxiao Wang, Zhe Sun, Ziqun Cao 期刊:Hans Journal of Ophthalmology 影響因子:1.58 PMID:

 

DMEM高糖 細(xì)胞培養(yǎng) solarbio